Growing MDCK Cells on Coverslips

[mutant11]

Hi all,

Does anyone work with MDCK cells? I want to seed them onto glass coverslips and have been using glass coverslips coated with poly-L-lysine. The cells sometimes do not stick well to the coverslips and I have found that the transient transfection rate is lower on the coated glass slides than just doing the transfections in the plastic cell culture wells.

How do you usually seed MDCK cells onto coverslips? Do you just use the coverslips alone? Do you add collagen? (how much etc)

Thanks!

[Jessica888]

It is most likely the coating of your coverslips is not good. The lower transfection rate is due to the lower survival rate of your transfected cells on poor coating. I know someone uses coated coverslips from neuvitro.com and got good culture results.