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DNA Extraction/Isolation

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DNA Extraction/Isolation

Postby Melstmu2010 » Thu Jun 30, 2011 8:31 pm

At the end of DNA extraction, heat at a temperature of 55 degrees Celsius is applied to Digestion buffer/Proteinase K solution. Then, at the end of DNA Isolation DNA/TE buffer solution is heated to 65 degrees Celsius. Why can't both reactions be heated at either 55 or 65 degrees Celsius? Why is there a 10 degree increase in temperature during DNA Isolation?
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Postby JackBean » Fri Jul 01, 2011 3:08 pm

I've never heated TE buffer with DNA. What step are you talking about?

You're adding some enzyme, right? Like RNase. Both heating steps serve to denature and inactive present enzymes.

Cis or trans? That's what matters.
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Postby vinayaksabnis » Sat Jul 09, 2011 3:06 pm

No idea but never heard for dna extraction we must heat it... Whats TE buffer u talking about Tris EDTA Ethylene Diamide Tetra Acetic acid???...According to me For Dna extraction we must carry out process in cold temperate zones...
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