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DNA Extraction/Isolation

Debate and discussion of any biological questions not pertaining to a particular topic.

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DNA Extraction/Isolation

Postby Melstmu2010 » Thu Jun 30, 2011 8:31 pm

At the end of DNA extraction, heat at a temperature of 55 degrees Celsius is applied to Digestion buffer/Proteinase K solution. Then, at the end of DNA Isolation DNA/TE buffer solution is heated to 65 degrees Celsius. Why can't both reactions be heated at either 55 or 65 degrees Celsius? Why is there a 10 degree increase in temperature during DNA Isolation?
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Postby JackBean » Fri Jul 01, 2011 3:08 pm

I've never heated TE buffer with DNA. What step are you talking about?

You're adding some enzyme, right? Like RNase. Both heating steps serve to denature and inactive present enzymes.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby vinayaksabnis » Sat Jul 09, 2011 3:06 pm

No idea but never heard for dna extraction we must heat it... Whats TE buffer u talking about Tris EDTA Ethylene Diamide Tetra Acetic acid???...According to me For Dna extraction we must carry out process in cold temperate zones...
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