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History of Polymerase Chain Reaction

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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History of Polymerase Chain Reaction

Postby PhdSparrow » Thu May 12, 2011 6:41 pm

I need articles about history of pcr. Could anybody share?
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Postby angel92 » Fri May 13, 2011 2:37 pm

i hope so, this video will help you
http://www.youtube.com/watch?v=_YgXcJ4n-kQ
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Postby angel92 » Fri May 13, 2011 2:40 pm

and
More than 30 years ago, the introduction of recombinant DNA technology as a tool for the biological sciences revolutionized the study of life. Molecular cloning allowed the study of individual genes of living organisms; however this technique was dependent on obtaining a relatively large quantity of pure DNA. This depended on the replication of the DNA of plasmids or other vectors during cell division of microorganisms (1). Researchers found it extremely laborious and difficult to obtain a specific DNA in quantity from the mass of genes present in a biological sample (2). Recombinant DNA technology made possible the first molecular analysis and prenatal diagnosis of several human diseases. Fetal DNA obtained by amniocentesis sampling could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4).
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Re:

Postby canalon » Sat May 14, 2011 3:02 am

angel92 wrote:and
More than 30 years ago, the introduction of recombinant DNA technology as a tool for the biological sciences revolutionized the study of life. Molecular cloning allowed the study of individual genes of living organisms; however this technique was dependent on obtaining a relatively large quantity of pure DNA. This depended on the replication of the DNA of plasmids or other vectors during cell division of microorganisms (1). Researchers found it extremely laborious and difficult to obtain a specific DNA in quantity from the mass of genes present in a biological sample (2). Recombinant DNA technology made possible the first molecular analysis and prenatal diagnosis of several human diseases. Fetal DNA obtained by amniocentesis sampling could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4).


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Postby PhdSparrow » Sat May 14, 2011 6:42 pm

I found good one.. Thanks for your comments. you can read : http://polymerasechainreaction.org/hist ... -reaction/
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Postby angel92 » Tue May 17, 2011 3:35 pm

PhdSparrow this is much better about the history of PCR
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