simple epitope

[derChronostat]

Hi,

I couldn´t rally figure out where to post this question.
Does anybody know an antibody that recognizes a very simple epitope - like a single loop? For example a peptide-antibody or an antibody binding to a single "site" on a protein.
Most of the antibodies recognize a context of several subepitopes which is too complicated for my approach.

Thanks in advance,

Chris

[JackBean]

you should be able to to raise antibodies only against your epitope (understand - use only that epitope for imunization), or raise polyclonal antibodies and lok, which one will best bind your epitope

[derChronostat]

Hi,

thanks for your reply.

this is not what the thing is about. I need something like a positive control - a known antibody known to bind a very simple epitope (best would be a loop - hence a nonlinear peptide or protein-fragment) which can be used in in-vitro experiments.

Cheers,
Chris

[JackBean]

I'm sorry, I don't understand.

[Darby]

I learned that there is a lower limit to the size of any potential epitope - is that what this is about-?

[canalon]

Epitopes are more than just a loop or any other shape since they include the shape of each aa in the sequence that makes that loop (or other).
I am not sure what you want to do but you can order specific peptides made by a number of companies, and some will also sell Ab specifically targeting those peptides. Would that be what you are looking for?