Login

Join for Free!
118302 members


rabbit ant-rat MCP1 prep

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

rabbit ant-rat MCP1 prep

Postby fluffy-sama » Fri Jul 22, 2005 6:46 pm

Um... This may be more of a chemistry question than anything else, but here goes.

I have 500 ug of purified rabbit IgG which I need to make .1% sodium azide. The sodium azide solution I have is 10%. How much sodium azide do I have to add to make the entire solution .1% sodium azide?
fluffy-sama
Garter
Garter
 
Posts: 1
Joined: Fri Jul 22, 2005 6:43 pm

Postby canalon » Sat Jul 23, 2005 2:04 am

.1% means that for 100ul (it could be ml or liters, but I suppose that you do not dilute that much you IgG) of solution you must have .1ul of it should be pure azide solution.

In your case that means that you should mix 1ul of 10% azide to 99ul of Ig solution.

Patrick
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest