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AS-PCR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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AS-PCR

Postby sali213 » Sun Dec 26, 2010 10:31 am

Hellow,
please i would know th protocol of AS-pcr and what is th difference between it and ASO-pcr.
thanks.
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Postby Kezzer » Mon Dec 27, 2010 1:41 pm

As far as I am aware AS-pcr and ASO-pcr are the same technique and use allele specific oligonuleotides (primers) in SNP detection.
There are plenty of protocols available online and on Springerlink for allele specific PCR.
BSc (Hons) Forensic Science,
MSc Biological Sciences
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Postby vbbgold » Mon Dec 27, 2010 3:38 pm

I agree with Kezzer

Here are the links:

http://en.wikipedia.org/wiki/Allele-spe ... nucleotide
http://www.springerlink.com/content/l03 ... 042&page=1

Hope it helps!!!
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Postby sali213 » Mon Dec 27, 2010 7:45 pm

thank's
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