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IC50

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IC50

Postby DivyaG » Wed Dec 01, 2010 8:47 pm

hey IC50
Last edited by DivyaG on Mon Dec 06, 2010 10:25 am, edited 1 time in total.
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Postby andresgutierrez » Thu Dec 02, 2010 7:28 pm

Hello,

You should plot a graph - X axis - concentration of drug and Y- axis - cell viability - ( Abs ( with drug) / Abs(control without drug addition ) *100%

So you can estimate Ic 50 as a concentration of drug at 50% position on Y axis.

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Postby JackBean » Thu Dec 02, 2010 8:43 pm

if you're talking about enzymes, you're looking for K_i, not IC50

there are several ways how to determine this value. You can either use simple v (speed of reaction) on [S] (as Michaelis-Menten graph), or Lineweaver-Burg (1/v on 1/[S]) or plenty of others (e.g. Dixon's, Hanes-Wolf, Wolf-Augustinson-Hoffsteeh, Hunter-Dowson etc)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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