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why do RNA show smear instead of discrete bands?

Genetics as it applies to evolution, molecular biology, and medical aspects.

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why do RNA show smear instead of discrete bands?

Postby tracyliu » Sat Oct 09, 2010 11:02 pm

if the isolated DNA is not treated RNase, then RNA are present, but how come RNA molecules don't get separated like the digested DNA fragments? aren't they also in different sizes?
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Postby sorin » Sun Oct 10, 2010 8:16 am

They get separated like DNA, but remember how many different RNA molecules are in a cell,its not like chromosomes or fragments of restriction digests having a discrete size. Apart from this if you do not carefully prepare your samples RNAse contamination can occur quite often...
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Postby JackBean » Sun Oct 10, 2010 10:27 am

as sorin wrote, there are plenty of RNAs in the cell each with different length (even the polyA can only differ)

Cis or trans? That's what matters.
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