About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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Is anyone using Veal Infusion Agar and/or broth for culturing anything? What/why?
I have a substantial amount of dehydrated Veal Infusion media left over from my predecessor. I'm in a collegiate lab, growing about 20 assorted bacteria for students to use/test, and I currently use TSA/TSB as my standard growing media (although I do have a few "problem children" that grow very slowly - Pseudomonas, Lactobacillus, Mycobacterium). If Veal Infusion would work better for these few tricky bacteria, then I'll keep it around and experiment with it - otherwise I don't want to open the new bottles so that they can find a new home.
Also, I have a ton of Lauryl Tryptose Broth, unopened. Is anyone using this - for what/why?
The Difco manual ( http://www.bd.com/ds/technicalCenter/in ... Manual.asp ) is your friend in that case. Most commercially available media are described and referenced with good comments on what it is generally used for and how to use it.
I am surprised that Pseudomonas are one of your problem children, but Veal infusion being richer than TSA or LB might provide what they need.
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
The Difco manual is my friend in EVERY case! I checked the manual already to know what it "should" be good for - I'm more wondering if anyone actually uses it in real life and what made them go with that medium.
We try to stay away from pathogens whenever there is a similarly-behaving alternative, so we use Pseudo fluorescens rather than auriginosa. I hear that auriginosa grows wonderfully - but the fluorescens just doesn't like to produce a dense growth very fast (overnight). When the students practice staining, they are usually searching around a bit on the slide to find it...unless I give it an extra day or two of grow time...but then of course it can be trickier to stain/stains unevenly. Any suggestions?
4 posts • Page 1 of 1
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