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sugestion pls

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sugestion pls

Postby sarguna » Thu Sep 02, 2010 5:23 am

i am doin an project related to bioplastic production in plants. i have an source gene from an microbe. in order to avoid the codon optimiztion procedure i am tinkin to go for plastid transformation. will this method will be useful for me/? for this method whether i should go for agrobacterium mediated or biolistic method? pls help me out.
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Postby JackBean » Thu Sep 02, 2010 9:08 am

To my knowledge, Agrobacterium infectes gDNA and biolistic method has largely decreased proficiency for plastids, but I'm sure there are some methods, just look into some papers ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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