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Transcription

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Transcription

Postby ACyanNight » Sun Aug 29, 2010 7:21 pm

Since RNA is transcribed by complementary pair matching, and DNA is double stranded, does that mean that only one side is used each time? Since the other side would result in a different sequence of bases? If so, how does the RNA polymerase know which side, and does that mean that only one strand is actually used, and the other strand is purely for replication purposes?
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Postby JackBean » Sun Aug 29, 2010 7:43 pm

Basically yes to all your questions ;) But both strands can be used for transcription, but only one each time ;)
And the RNA Pol recognize it by binding to specific sequence
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Transcription

Postby ACyanNight » Sun Aug 29, 2010 9:48 pm

Hm.... Say you have a strand of DNA that goes

ATGCAGTCGACCGAT
TACGTCAGCTGGCTA

So, the two possible mRNA's would be

UACGUCAGCUGGCUA

AUGCAGUCGACCGAU

The sequences would obviously result in different amino acid sequences, right? So, would both create working proteins? Or would only one?
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Postby sorin » Mon Aug 30, 2010 11:55 am

transcription initiation only occurs at the strand that is defined by specific sequence patterns, as jackbean already mentioned. At the key of this patterns are promotors, which lie 5' upstream of each gene. Promotors mediate interactions between DNA binding proteins and the RNA polymerase, whereby transcription is initiated. So of course will the transcription of both strands results in different polypeptides, but it is important to consider if both strands are actually transcribed inside the cell...
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Re:

Postby JackBean » Tue Aug 31, 2010 3:29 pm

sorin wrote:Promotors mediate interactions between DNA binding proteins and the RNA polymerase, whereby transcription is initiated.


Well, actually DNA binding proteins mediate the interaction between DNA (in form of promotor) and RNA Pol ;)
http://www.biolib.cz/en/main/

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