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Precipitation of BSA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Precipitation of BSA

Postby dhkwak » Sun Mar 14, 2010 9:06 am

Hey guys,

I have precipitated BSA using by:

(i) adding cold, dry acetone in 5 or more quantities per protein solution,
(ii) incubating at -20 Celsius for at least an hour, and
(iii) centrifuging at 13,000 g for 15 min and removing the supernatant.

I wanted to know if anyone has had issues with using the acetone precipitation method for BSA. I had read that BSA can be effectively precipitated using ammonium sulfate (I am uncertain of the concentrations but have read usually 75% will suffice). Does the acetone method result in aggregation? Any help would be greatly appreciated! Thanks
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Postby JackBean » Tue Aug 10, 2010 10:46 am

I think it shouldn't, if you add it slowly. But that's why I like more using ammonium sulphate instead of organics...
http://www.biolib.cz/en/main/

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