Dissociation Constants

[pmngrad]

Quick question of clarification,

If a particular receptor has a dissociation constant (Kd) of 50nM for instance. That means that the receptor is half bound by ligand when the concentration of the ligand is 50nM.

To fully saturate a receptor, would one use twice as much ligand? 100nM in this case?

Thanks for your help with this....

All the best

[JackBean]

not really, it's similar as Michaelis with enzymes, so you won't get twice so much bound ligands

Usually, 10× more is taken to fully saturate