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the mannitol concentration

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the mannitol concentration

Postby Dong » Wed May 05, 2010 12:12 pm

Is there anyone could tell the contration of mannitol that equal to 20mM K2SO4 added to the agar plate ?

and the fomula ion stress equal to the mannitol concentration ?


thanks for help !
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Re: the mannitol concentration

Postby Dong » Fri May 07, 2010 12:49 am

Is there anyone could help me ? :cry: :cry:
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Postby Jillo725 » Fri May 07, 2010 1:04 am

i hate plants. =p
"There is no adequate defense, except stupidity, against the impact of a new idea."
— Percy Williams Bridgman, US physicist
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Postby JackBean » Fri May 07, 2010 7:15 am

how can you compare manitol and potassium sulphate? One form three ions, whereas the other is neutral and could serve as carbon source
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby Dong » Sun May 09, 2010 1:51 am

the use of mannitol is just intended to check wether the effect of ions addition is caused by the increased water pressure ?
So, I want to know
the phenomen (addition 20mM K2SO4 cause plant grows poor) is because of water pressure enhanced ?
But the problem is the concentration that equals to 20mM K2SO4 .

20mM K2SO4 = ? mannitol

Thanks !
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Postby Dong » Thu May 13, 2010 5:10 am

some friends told me ,
in solution, 20mM K2SO4 = 40 or 60 mM Mannitol
but they can not offer the souce !

I don't know whether that is right .

anyone could tell me ?

Thanks
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Postby JackBean » Thu May 13, 2010 10:49 am

Look to Vyroubalová et al. 2009; we have used PEG, but in Materials and Methods is reference to article, from which we took the concentration, there may be also mannitol.

However, as I said, mannitol will influence the plant growth by itself, so if you want to see only the effect of osmotic pressure, you should use something inactive.
http://www.biolib.cz/en/main/

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