Type ii restriction endonucleases

[Soni]

Does anybody know how three different type ii restriction endonucleases can cut at the same sequence but at different points to yield three different set of products?

[JackBean]

That's pretty simple. The ER can produce either blunt ends or sticky ends with either 5'- or 3'-overhang. Thus if you had sequence GAATTC you can have:

G...AATTC
CTTAA...G

GAA...TTC
CTT...AAG

GAATT...C
C...TTAAG