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Loading control for different human tissue samples

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Loading control for different human tissue samples

Postby samkh918 » Tue Apr 13, 2010 11:10 pm

Hello,

I have ordered an immunoblot (NC membrane) for western blotting, which contains different human tissues such as kidney, spleen, prostate, etc. in each lane. I want to see if equal amount of proteins have been loaded in each lane as promised by the company but my problem is that I can't use B-tubulin as the loading control since different tissue express different levels of this protein.
I was wondering what would be the best way for this.

Thank you in advance for your reply.
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Postby MrMistery » Mon Apr 19, 2010 1:15 am

So wait someone else ran a gel and blotted it, and then just sent you the membrane? Jesus, what would they think of next?
Anyway, maybe PGK? Actin?
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