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Only for intelligent virologists

Debate and discussion of any biological questions not pertaining to a particular topic.

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Only for intelligent virologists

Postby hashemyemen » Tue Mar 23, 2010 6:15 pm

suppose that we isolated the viral genome from two different species of viruses..

The fist genome will be isolated from a bacteriophage.

The second genome will be isolated from TMV.


Then we inserted both genomes into bacteria
, now which genome will be expressed, The one of TMV, or that one of Bacteriophage?? And what will be the result??

Of course, it is just a question, It is not means that I have done this before, but it is just a test for you... and me as well :D

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Postby vet0lover » Tue Mar 23, 2010 7:01 pm

well I think if bacteriophage is specific for TMV
genome so bacteriophage will engulfed TMV
But if It's not specific may be TMV will show It's charcter
I really hope to do that in lab so I can tell u the accuret
result
thx 4 good question
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Re:

Postby hashemyemen » Wed Mar 24, 2010 1:37 am

vet0lover wrote:well I think if bacteriophage is specific for TMV
genome so bacteriophage will engulfed TMV
But if It's not specific may be TMV will show It's charcter
I really hope to do that in lab so I can tell u the accuret
result
thx 4 good question


If you can do it in your Lab, that will be great....
I really have not tried to do it before, but let me guess about it :

I think that there will be a competition b/t both DNAs, only one of them will be expressed, and only a single type of virus will start producing its self..

According to my knowledge, bacteriophage has much more chances to be express its genome more than chances for TMV, the reason is that TMV in most cases infect the plant cells and express its genome there...

Hmmmm, it will be good if you try it practically...


The another question raises, that What will be happened if you try it with box-virus where both bacteriophage and box-virus are animal viruses which can infect prokaryotic and eukaryotic??

Look to the picture where the other virus is Box type of viruses..
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Postby mith » Wed Mar 24, 2010 1:43 am

tmv has not been shown to have any effect on anything other than plants, so the chances are more like zero. most likely the ribosome and other protein machinery are not compatible.
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Re:

Postby hashemyemen » Wed Mar 24, 2010 4:13 am

mith wrote:tmv has not been shown to have any effect on anything other than plants, so the chances are more like zero. most likely the ribosome and other protein machinery are not compatible.




As I mentioned...

Now, what will be the case when TMV replaced by box-virus??
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Postby mith » Wed Mar 24, 2010 11:14 pm

Why don't you specify a bacteria as well? If this was meant to be a thought experiment, I think you've already made your point. If it's meant to be experimental, I'm not sure how to answer that.
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Re:

Postby hashemyemen » Thu Mar 25, 2010 1:59 am

mith wrote:Why don't you specify a bacteria as well? If this was meant to be a thought experiment, I think you've already made your point. If it's meant to be experimental, I'm not sure how to answer that.


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Postby vet0lover » Fri Mar 26, 2010 7:53 pm

well as I mentioned i'm NOT a prof but I think that there's no VIRUS
called Box virus (exp. computer virus)
But if ur ques abt Pox virus I think after competition b/t Pox & bacteriophage genomes
in E.coli bacteriophage will be the winner as bacteriophages like T4 bacteriophage with its six legs as example will burst this E.coli piece of cake
well this is my Answer so what's ur opinion?????????????
I'm waiting on fire
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Re:

Postby hashemyemen » Sat Mar 27, 2010 6:36 am

vet0lover wrote:well as I mentioned i'm NOT a prof but I think that there's no VIRUS
called Box virus (exp. computer virus)
But if ur ques abt Pox virus I think after competition b/t Pox & bacteriophage genomes
in E.coli bacteriophage will be the winner as bacteriophages like T4 bacteriophage with its six legs as example will burst this E.coli piece of cake
well this is my Answer so what's ur opinion?????????????
I'm waiting on fire


Yes, your answer is right, but next time it will be better to explain your answers....

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Postby vet0lover » Sat Mar 27, 2010 6:50 pm

Thx alot
hashemyemen
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Re:

Postby hashemyemen » Sun Mar 28, 2010 4:29 am

vet0lover wrote:Thx alot
hashemyemen


You are welcome :wink:
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