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I need immediate help!! How to PCR primer designing?

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I need immediate help!! How to PCR primer designing?

Postby veniesh » Sun Aug 16, 2009 8:54 am

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Last edited by veniesh on Tue Nov 03, 2009 2:03 pm, edited 1 time in total.
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Postby jyaron » Sun Aug 16, 2009 6:32 pm

There's a primer designer that's highlighted in this blog post:

http://thebioblog.com/2009/08/5-great-o ... iologists/
Experience: Cell Biology, Confocal Microscopy, Developmental Biology, Evolutionary Biology, Genetics, Genomics, Physiology
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Postby priyajoshi » Mon Oct 19, 2009 8:26 pm

http://bioinfoman.com/bxaf5/bm/bm_seq_primer.php
Please check this out.
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Postby amymiller » Tue Oct 20, 2009 6:22 pm

I will recommend BxSeqTools designed by Bioinforx.
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Re: I need immediate help!! How to PCR primer designing?

Postby JackBean » Fri Oct 23, 2009 12:02 am

veniesh wrote: The sequence of sense primer A was 5’-GA(C/T) GA(C/T) III T(T/A)(T/C) GA(C/T) GCI (C/T)A(C/T) GG-3’.

My question is how to write the sequence of sense primer A from conserved amino acid sequence motif primer A ??

I really don't get, what's your problem? :?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby martao » Fri Feb 26, 2010 11:39 am

You can also use blast primer design tool. Just google it out. It's easy and gives you a lot of options to choose from.
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