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Do someone know SH-SY5Y cells??

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Do someone know SH-SY5Y cells??

Postby Sohye » Mon Feb 22, 2010 2:48 am

I am culturing several types of cell.
BUT One of them, SH-SY5Y, has a problem :(
It didn't stick to the surface of culture dish.
I used to a mixture of DMEM, FBS and penicillin and the cells are always grown at 37degrees Celsius with 95% air and 5% carbon dioxide.
What's something wrong??
Help meeeeeeeeeeeeeeeeeeeeeeeeeee please :(
Sohye
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Re: Do someone know SH-SY5Y cells??

Postby violsk » Wed Feb 24, 2010 6:53 am

Hi!
I am using SH-SY5Y since a long time, for my graduation thesis.
Have you tried to work with polylysinated dishes? with this simple method I solved all my problem of non attached cells.
As Medium it is suggested to use F12:MEM 1:1, FCS(or FBS) 10%, Pen/Strep 2%, Glutamin 200mM, Na-pir 100mM.
Hope to have been useful,
have a good work!!
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Postby albinoblacksheep » Wed Mar 09, 2011 5:07 am

my SY5Y's have problems at the moment too. They were low confluency, so I just changed the media, and half hour later, most of them had died :( (I used the same media as always...) any ideas? has this happened to anyone before?
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