Do someone know SH-SY5Y cells??

[Sohye]

I am culturing several types of cell.
BUT One of them, SH-SY5Y, has a problem
It didn't stick to the surface of culture dish.
I used to a mixture of DMEM, FBS and penicillin and the cells are always grown at 37degrees Celsius with 95% air and 5% carbon dioxide.
What's something wrong??
Help meeeeeeeeeeeeeeeeeeeeeeeeeee please

[violsk]

Hi!
I am using SH-SY5Y since a long time, for my graduation thesis.
Have you tried to work with polylysinated dishes? with this simple method I solved all my problem of non attached cells.
As Medium it is suggested to use F12:MEM 1:1, FCS(or FBS) 10%, Pen/Strep 2%, Glutamin 200mM, Na-pir 100mM.
Hope to have been useful,
have a good work!!

[albinoblacksheep]

my SY5Y's have problems at the moment too. They were low confluency, so I just changed the media, and half hour later, most of them had died (I used the same media as always...) any ideas? has this happened to anyone before?