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Paraffin section restaining

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Paraffin section restaining

Postby Jiippuuts » Fri Feb 19, 2010 1:25 pm

Hi,

Making a quite important series of stainings. Of course used the wrong secondary antibody. The species is right (rabbit), but it's not biotinylated although it should... Beginners mistake. Can I use the same sections again with comparable results? They are now in PBS in +4. At which point of the protocol I should start the staining again? Incubation with the correct secondary? Or has the wrong secondary antibody already attached to all of the "rabbity parts" in my sections...

Grateful for any help.
Jiippuuts
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