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synchronising cell cultures

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synchronising cell cultures

Postby kk » Tue Feb 02, 2010 10:40 am

I would like to synchronise my cells (embryonic fibroblasts) so that when I fix cells after coming out of the arrest no cells will be going into or just coming out of mitosis. I want to avoid apoptosis though, basically I want to make sure at the time of fixation all my cells are interphase cells. Any suggestions?

p.s. after cells had possibly rounded up from nocodozole treatment - not from apoptosis yet - will they be able to reattach?
kk
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Postby MrMistery » Thu Feb 04, 2010 10:38 pm

we used to starve them of serum to synchronize them. can't you just do that?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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Postby kk » Tue Feb 09, 2010 2:39 pm

I could try serum starvation... tx!
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