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Debate and discussion of any biological questions not pertaining to a particular topic.

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Postby sixpence » Sat Jan 30, 2010 5:21 am

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Last edited by sixpence on Thu Feb 18, 2010 3:03 am, edited 1 time in total.
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Postby mkwaje » Sat Jan 30, 2010 11:14 am

Do I understand that you are trying to make a plate with just lactose as C source? Well, you have to add other ingredients like N source unless your yeast is a N-fixer, it cannot get N out of the air, even then pure lactose and agar won't likely support your yeast... You can use minimal medium and there are some media without C source. There are chemically defined media used to determine the C source requirement of unknown bacteria. You can use that.

hope that helps.
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Re: Can you grow yeast on plates containing only lactose?

Postby sixpence » Sat Jan 30, 2010 10:17 pm

Well I wanted to make something where a yeast species w/o ability to metabolize lactose would not grow and the bacteria could grow. I didn't want to just grow yeast, if that makes sense.
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Postby JackBean » Sat Jan 30, 2010 10:45 pm

than use some minimal media and as carbon source use lactose.
But bacteria and yeast have quite distinct needs, so already the choose of media will be discriminating ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Can you grow yeast on plates containing only lactose?

Postby sixpence » Sun Jan 31, 2010 2:52 am

So it would easier to just grow them on plates with xgal and only the bacteria plates should be blue?
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Postby JackBean » Sun Jan 31, 2010 3:17 pm

why should it be blue? Do you have some transformed bacteria? What are you doing?
http://www.biolib.cz/en/main/

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Postby mkwaje » Sun Jan 31, 2010 5:10 pm

I don't understand what you are trying to do. If you want to select for yeasts that can metabolize lactose, then do what JackBean said. Use minimal medium + lactose as the sole C source.

Then you said you also wanted to grow bacteria on it? Meaning you have a mixed population at the start? For bacteria, you have to have some idea on its nutrient requirements not just the ability to break down lactose. Another thing is that once lactose is broken by enzymes released in the environment. You'll get small colonies around it that will also be growing although they cannot metabolize lactose. These small colonies can grow because the lactase enzymes are extracellular thereby resulting to the presence of glucose which almost all bacteria/yeast can utilize.

Historical studies also suggest that bacteria (or maybe even yeast) can "adapt" to the presence of lactose as the sole C source. - http://www.jstor.org/pss/82643
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Re: Can you grow yeast on plates containing only lactose?

Postby sixpence » Sun Jan 31, 2010 6:41 pm

sorry for being unclear. so i just want a hypothetical way to identify yeast from bacteria (ecoli) without looking at colony morphology or anything, assuming that ecoli has beta lactamase and yeast does not. at first i thought it would be fine to use minimal media with lactose to identify the two but you guys brought up the extra nutritional requirements so that probably wouldn't work. so then i thought why not use the blue white screen... does that make sense now?
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