Biology-Online • View topic - bacteriophages

bacteriophages

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

bacteriophages

Draw fully labelled diagram, and write some explanatory notes, explaining how you would quantify the number of bacteriophages in an unknown suspension.
taata
Garter

Posts: 11
Joined: Mon Apr 25, 2005 10:10 pm

you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.

mothorc
Coral

Posts: 138
Joined: Sat May 21, 2005 2:27 pm
Location: Farfaraway country

mothorc wrote:you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.

There are easier solutions, that do not need any microscope: search for plaque assay? counting.

canalon
Inland Taipan

Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm

year

terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)

mothorc
Coral

Posts: 138
Joined: Sat May 21, 2005 2:27 pm
Location: Farfaraway country

Re: year

mothorc wrote:terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)

First, related to the question at the beginning of the thread, I do not think you have to take this into account: He's talking about bacteriophage, You just a sensitive strain of bacteria, the one used for propagating the phage would be good
Then if you cannot use plaque, your method seem good to me, although a real pain to prepare compared to the other...

Patrick

canalon
Inland Taipan

Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm

ok ok I see

mothorc
Coral

Posts: 138
Joined: Sat May 21, 2005 2:27 pm
Location: Farfaraway country