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HMGCoA Reductase

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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HMGCoA Reductase

Postby redmerlin » Wed Jul 13, 2005 10:27 am

Hi,
I am trying to check the levels of HMGCoA Reductase gene expression in rats that i've dosed with a drug designed to decrease cholesterol levels.
I used TriZOL to extract mRNA from hepatocytes, then did an RT-PCR & got cDNA. I chkd the presence of cDNA by amplifying the G6PD gene in the sample. But now i am unable to amplify th HMG gene. The primers r correct. I tried various conc of MgCl2 and used a temp gradient, but it dindt work.
Can ny1 plz tel me what the problem is?
Thanks. All help is appreciated.
redmerlin
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