Login

Join for Free!
114112 members


Deproteinizing plasma?

For discussing the functions of different structures of all organisms.

Moderator: BioTeam

Deproteinizing plasma?

Postby ntb103 » Mon Jan 18, 2010 5:48 pm

Does anyone have a step by step protocol for deproteinizing plasma for glucose with barium hydroxide and sulfate?
ntb103
Garter
Garter
 
Posts: 4
Joined: Mon Jan 18, 2010 5:44 pm

Postby JackBean » Mon Jan 18, 2010 7:33 pm

for glucose?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5657
Joined: Mon Sep 14, 2009 7:12 pm

Re: Deproteinizing plasma?

Postby biohazard » Tue Jan 19, 2010 7:35 am

If you deproteinize plasma theres nothing much left :(

All you got left is some glucose, ions, non-protein hormons and such... and a lot of water.
User avatar
biohazard
King Cobra
King Cobra
 
Posts: 776
Joined: Sun Apr 08, 2007 6:45 pm


Re: Deproteinizing plasma?

Postby ntb103 » Tue Jan 19, 2010 10:50 am

Yes, for glucose!! Does anyone have a protocol?
ntb103
Garter
Garter
 
Posts: 4
Joined: Mon Jan 18, 2010 5:44 pm

Postby JackBean » Tue Jan 19, 2010 12:38 pm

He's talking about plasma, not plasma membrane! :lol: :twisted:

Sorry, I don't get, what you mean by "for glucose"
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5657
Joined: Mon Sep 14, 2009 7:12 pm

Re: Deproteinizing plasma?

Postby ntb103 » Tue Jan 19, 2010 2:15 pm

Yes, blood plasma. I need to infuse radiolabelled glucose in vivo and measure hepatic glucose production. I am just not sure the best way to deproteinize the blood to get the infused glucose vs. blood glucose values?
ntb103
Garter
Garter
 
Posts: 4
Joined: Mon Jan 18, 2010 5:44 pm

Postby JackBean » Tue Jan 19, 2010 2:40 pm

What scale do you need? How many samples? Do you need it online?

What about to try desalting column of gel filtration?

EDIT
or you can try precipitation, either by ammonium sulphate, organic solution, or probably most appropriate for you by heat.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5657
Joined: Mon Sep 14, 2009 7:12 pm

Re: Deproteinizing plasma?

Postby ntb103 » Tue Jan 19, 2010 5:24 pm

Working with 10 microL of plasma per sample and about 10 samples per run. I am not sure what you mean by online?
ntb103
Garter
Garter
 
Posts: 4
Joined: Mon Jan 18, 2010 5:44 pm

Postby JackBean » Wed Jan 20, 2010 4:03 pm

By online I meant something like taking blood from someone,measure and probably give back...

Well, with 10 ul, you can use some spin-filter or the precipitation...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5657
Joined: Mon Sep 14, 2009 7:12 pm

Postby bdastiarraga » Wed Nov 23, 2011 2:30 pm

have you found the protocol for blood glucose?
bdastiarraga
Garter
Garter
 
Posts: 1
Joined: Wed Nov 23, 2011 2:25 pm


Return to Physiology

Who is online

Users browsing this forum: No registered users and 1 guest

cron