Deproteinizing plasma?

by **ntb103** » Mon Jan 18, 2010 5:48 pm

Does anyone have a step by step protocol for deproteinizing plasma for glucose with barium hydroxide and sulfate?

by **JackBean** » Mon Jan 18, 2010 7:33 pm

for glucose?

by **biohazard** » Tue Jan 19, 2010 7:35 am

If you deproteinize plasma theres nothing much left :(

All you got left is some glucose, ions, non-protein hormons and such... and a lot of water.

by **ntb103** » Tue Jan 19, 2010 10:50 am

Yes, for glucose!! Does anyone have a protocol?

by **JackBean** » Tue Jan 19, 2010 12:38 pm

He's talking about plasma, not plasma membrane! :lol:

Sorry, I don't get, what you mean by "for glucose"

by **ntb103** » Tue Jan 19, 2010 2:15 pm

Yes, blood plasma. I need to infuse radiolabelled glucose in vivo and measure hepatic glucose production. I am just not sure the best way to deproteinize the blood to get the infused glucose vs. blood glucose values?

by **JackBean** » Tue Jan 19, 2010 2:40 pm

What scale do you need? How many samples? Do you need it online?

What about to try desalting column of gel filtration?

EDIT
or you can try precipitation, either by ammonium sulphate, organic solution, or probably most appropriate for you by heat.

by **ntb103** » Tue Jan 19, 2010 5:24 pm

Working with 10 microL of plasma per sample and about 10 samples per run. I am not sure what you mean by online?

by **JackBean** » Wed Jan 20, 2010 4:03 pm

By online I meant something like taking blood from someone,measure and probably give back...

Well, with 10 ul, you can use some spin-filter or the precipitation...

by **bdastiarraga** » Wed Nov 23, 2011 2:30 pm

have you found the protocol for blood glucose?

Deproteinizing plasma?

Deproteinizing plasma?

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