Any experience of MEFS

[Chunshijia]

Hello:

Now I want to set up a serise of experiments as follows:

1.MEFs from Gene X KO mouse;
2.Lentivirus/Retrovirus of oncogene RAS infectionof MEFS;
3.Then the MEFS will be injected into nude mice;

My questions are:

1.If MEFS can be infected by lentivirus/retrovirus;
2.If any experts can provide some detailed protocals or give me any experience of theirs!


Thank you very much!