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Coloured Filters on Colorimeter.

For discussing the functions of different structures of all organisms.

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Coloured Filters on Colorimeter.

Postby aaaaaaaaaa17 » Wed Nov 25, 2009 11:13 pm

Hey,
Does anyone know why you insert a blue filter in the colorimeter when using Nessler's reagent (Ammonia), but a red filter when using Benedict's (Uric acid) reagent?

Thanks you.
aaaaaaaaaa17
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Postby JackBean » Thu Nov 26, 2009 3:37 am

Because you measure the complementary wavelengths?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby jwalin » Thu Nov 26, 2009 4:05 pm

@aaaaaaaaa17
first think
what does colorimeter do?
what does it measure?
what color are the 2 solution.


and keep in mind if a substance is blue it absorbs all other light wavelengths except that of blue.
the other two basic colors, red and green are also absorbed
it isn't what you do that matters but it is how you do it
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