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Postby TheDude » Tue Aug 04, 2009 3:52 pm

Dear friends,

I am pretty new to micelle biology and have some...maybe dumb questions.
So, are there any easy ways to manufacture micelles with standard laboratory equipment? I think, if the technique is established, it shouldn't be a problem to insert some special gangliosides into the membrane, right? And third, is there a way to let them not fuse with eucaryotic cell membranes in micelle-lymphocyte co-cultures?

Thanks a lot for your support!

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Postby mith » Thu Aug 06, 2009 11:09 pm

No you need specialized equipment, last I heard one of the labs on campus was using a layer of lipids and then pushing thru the layer perpendicularly to create the micelles. Unfortunately it's a work in progress i.e. no details being divulged.
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Postby MrMistery » Fri Aug 07, 2009 2:30 am

Well I'm pretty sure that you can do it, though probably not with standard equipment. However, i do remember reading a paper a while back that showed bilayers can grow in vitro by accumulating micelles. So I guess you could just look up that kind of paper and then go crazy with the methods section.
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