Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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ok I know that I was borrrrrrrrrring about theme "protein folding" but couldn't leave it.
I was thinking about synthesis of human proteins by bacteria E. coli So as E. coli is bacterium it has different chaperones than humans so if it would be necessary to produce proteins with quartenary structure that woud ba a problem unless you also insert gene for proper chaperone in E. coli... cause if not the nonfunctional protein could be formed...What do you think? I'm just asking for opinion I don't bite you know
Last edited by zami'87. on Thu Aug 20, 2009 6:17 pm, edited 1 time in total.
Our local expert on E. coli, Canalon will probably have your answer
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
They live in the last part of the colon if i remember correctly
It is not. The problem is that victor saw the word 'E.Coli' and didn't read further...
It matters not how strait the gate
How charged with punishment the scroll
I am the Master of my fate
I am the Captain of my soul.
sorry.I wasn't angry. I just don't want to rename topic.
The answer from the local E. coli expert is that he doesn't play with those eucaryotic genes. And he wouldn't contaminate his nice pet bacteria with such horrors.
More seriously, it is indeed true that you cannot express all eucaryotic genes in bacteria. And that is exacly why you have so many diffrent eucaryotic expression systems (from insect cells to GM plants). And inserting just the proper chaaperone is just not enough, or you would have the strain going around if it was so simple. There are a lot of post translationnal modification that cannot be processed in bacteria.
Hope this helps (not lazy today, rather trying to avoid helping in the lab clean-up)
Thanks Patrick !
I know it's still a secret but in the future.....
The final EC stands for Escherichi coli as for the rest:
ET: Entero toxigenic
EI: Entero invasive
These are different type of pathogenic E. coli, it is a medical classification that reflects the main set of virulence genes in the bacteria and hence it's mode of infection. I think ther were more, but i do not find my references books to complete your list.
But again, what's the relation with the topic?
11 posts • Page 1 of 1
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