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Horse blood agar

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Horse blood agar

Postby sdrc0708 » Fri Apr 17, 2009 1:35 pm

I cultured an unknown bacteria on horse blood agar and got some colonies grown.
But how do you know if there is haemolysis?
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Postby Darwin420 » Sat Apr 18, 2009 12:41 am

Try silk screening...and transfer to a media with pure horse blood.
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Postby canalon » Sat Apr 18, 2009 2:24 am

you should see a halo around the colonies, see an exemple on that page (scroll down to the differences between alpha, beta and gamma hemolysis):
http://www.answers.com/topic/hemolysis
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Postby MrMistery » Sat Apr 18, 2009 9:42 pm

it has always been a mystery to me why microbiologists feel the need to refer to no hemolysis as a type of hemolysis.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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Re: Horse blood agar

Postby JorgeLobo » Sun Apr 19, 2009 9:55 pm

Horse blood agar can't be counted on to provide the same effects as is seen on sheep blood agar.

Why are you using the stuff?
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Postby Sepals » Mon Apr 20, 2009 8:57 am

Beta haemolysis is a clear area around the colonies.

Alpha haemolysis is a greenish area around the colonies.

There's also so called gamma, which is technically no haemolysis.
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Re: Horse blood agar

Postby Sepals » Mon Apr 20, 2009 9:00 am

JorgeLobo wrote:Horse blood agar can't be counted on to provide the same effects as is seen on sheep blood agar.

Why are you using the stuff?
Horse blood is more nutritious than sheep blood, which is why it is preferred in the UK. I used hourse blood on my MSc and it didn't affect haemolysis.
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Re: Horse blood agar

Postby JorgeLobo » Tue Apr 21, 2009 9:02 pm

I've not seen texts or publications that indicate horse blood agar - or confirm it's broad application as consistent with sheep blood. Can you provide these - as well as those demonstrating it to be "more nutritious'?
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Re: Horse blood agar

Postby ozboats » Thu Mar 04, 2010 4:57 am

A good comparisom of HBA , SBA and HuBA can be found by
F. M. Russell et. al.
at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594681/
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