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Transfection with more than one Plasmid

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Transfection with more than one Plasmid

Postby ybbobck » Sun Jul 03, 2005 12:22 am

If u transfect a mammalian cell line like 293 t with more than one plasmid , for instance one plasmid encodes for EGFP and the other encodes for E47, what are the chances that the 293 t cells take up ony one of the plasmid and not both.
If I vary the ratio of these 2 input plasmids..is there any chance that the cells take up just one plasmid...?
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