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Lactate Dehydrogenase (EC 1.1.1.27)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Lactate Dehydrogenase (EC 1.1.1.27)

Postby M. Mulder » Sun Jun 26, 2005 11:48 am

Hello,

I want to know how many isozymes there are for lactate dehydrogenase (EC 1.1.1.27)
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/enzymes/GetPage.pl?ec_number=1.1.1.27

I'm only interested in Homo Sapiens.
Thera are three PDB-files that contains the enzym-activity:
- 1i0z (chain A, B ) --> Homo-tetramer [syn. LDH-H, LDH-B, Heart sub-unit)
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1i0z
- 1i10 (chain A, B, C, D, E, F, G, H) --> Homo-tetramer [syn LDH-M, LDH-A, Muscle sub-unit]
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1i10
- 1t2f (chain A, B, C, D) --> Homo-tetramer [syn. LDH-H, LDH-B, Heart sub-unit
http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1t2f

If you navigate to the chains, for example the chains of 1i0z (http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1i0z&template=protein.html&l=1) and you navigate to the uniprot-file P07195 (http://www.ebi.uniprot.org/entry/P07195) you can see that the sub-units are a tetramer. But the 3D-display is a dimer. How is that possible?

I know that this enzym (EC 1.1.1.27) has at least 5 isozymes; LD1, LD2, LD3, LD4 and LD5. They differ in compound in 2 sorts of sub-units. H (=Heart) and M (=Muscle) In a tetramer you get the following variants:

HH
HH

HH
MH

HH
MM

HM
MM

MM
MM


How can I find back these iso-zymes? This link wil give you the information:
http://www.chclibrary.org/micromed/00054240.html
M. Mulder
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Postby M. Mulder » Wed Jun 29, 2005 9:28 pm

I'm actually want to know how many isozymes there are for the different enzymes we know of humans. But I think the given databases can't give me an answer to that. :cry:

Does anybody know how to find out ???

The databases only gives some information over the sub-units. I don't get it exactly.
M. Mulder
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