Login

Join for Free!
119218 members


Help Identifying Unknown Bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Help Identifying Unknown Bacteria

Postby nawahi » Sun Nov 23, 2008 7:20 am

I need a little help identifying my unknown.
It’s a strange color which I’d describe as being a tan white
(picture: http://i18.photobucket.com/albums/b136/Nawahi/image.jpg)
gram negative cocci.
This bacteria grows really quickly and the colonies are at a normal size.
Coagulase positive.
Peroxide: negative
It’s aerobic & Facultative.
I’ve done an entertube test which showed it had: glucose/gas, lactose and urea.
Antibiotics Test results:
Basetration 10 units (B-10): 12mm, defined ring
Tetres Cydramene (TE-30): 22mm, defined ring
Ampacylen (AM-10): 11mm, defined ring
Sipro (CF-30): 16 mm, defined ring
Sulfamatexizol (SXT): 23 mm, continuing growth
(P-10): 10mm, short resistance
Streptomyocin (S-10): 27 mm, defined growth
Rithromison (E-15): 36 mm, defined
D-30: 27 mm, grow back
CIP5: 30mm, grow back
nawahi
Garter
Garter
 
Posts: 4
Joined: Sun Nov 23, 2008 5:10 am

Postby stopherlogic » Sun Nov 23, 2008 9:00 am

Can you not do an API?

Have you tried looking in Bergeys manual?
stopherlogic
Death Adder
Death Adder
 
Posts: 80
Joined: Thu Sep 25, 2008 12:20 pm

Re:

Postby nawahi » Tue Nov 25, 2008 12:29 am

stopherlogic wrote:Can you not do an API?
I'm not sure what a API is.
Have you tried looking in Bergeys manual?

I've only tried once, but this is my first time trying to identify a bacteria and I'm not quite sure what I'm looking at in the manual, it all seams really confusing for me.
nawahi
Garter
Garter
 
Posts: 4
Joined: Sun Nov 23, 2008 5:10 am


Re: Help Identifying Unknown Bacteria

Postby nawahi » Tue Nov 25, 2008 9:44 pm

nawahi wrote:I need a little help identifying my unknown.
It’s a strange color which I’d describe as being a tan white
(picture: http://i18.photobucket.com/albums/b136/Nawahi/image.jpg)
gram negative cocci.
This bacteria grows really quickly and the colonies are at a normal size.
Coagulase positive.
Peroxide: negative
It’s aerobic & Facultative.
I’ve done an entertube test which showed it had: glucose/gas, lactose and urea.
Antibiotics Test results:
Basetration 10 units (B-10): 12mm, defined ring
Tetres Cydramene (TE-30): 22mm, defined ring
Ampacylen (AM-10): 11mm, defined ring
Sipro (CF-30): 16 mm, defined ring
Sulfamatexizol (SXT): 23 mm, continuing growth
(P-10): 10mm, short resistance
Streptomyocin (S-10): 27 mm, defined growth
Rithromison (E-15): 36 mm, defined
D-30: 27 mm, grow back
CIP5: 30mm, grow back
nawahi
Garter
Garter
 
Posts: 4
Joined: Sun Nov 23, 2008 5:10 am

Re: Help Identifying Unknown Bacteria

Postby nawahi » Tue Nov 25, 2008 9:48 pm

I finally found out what my unknow bacteria is. It's a: Listeria monocytogenes.
I ended up taking a new gram stain and looking at it under a microscope again, my results came out completely different than what I had previously: gram positive, diplobassili, with a capsule. I think what happened was the Listeria monocytogenes untilized the organism that I had growing previously and completely destroyed it and repopulated its self.
nawahi
Garter
Garter
 
Posts: 4
Joined: Sun Nov 23, 2008 5:10 am

Postby JorgeLobo » Sat Nov 29, 2008 5:18 pm

I have no idea how you "found out" but the results you offered are not consistent with Listeria monocytogenes. Your explanation is silly - but does indicate you probably did not have a pure culture. this is a critical element of any micro study and i suggest you concentrate on it.
JorgeLobo
Coral
Coral
 
Posts: 424
Joined: Mon Nov 17, 2008 12:12 am

Re: Help Identifying Unknown Bacteria

Postby dshenberger » Tue Dec 02, 2008 9:55 pm

NEED HELP! Unknown Bacteria.
Gram Pos Rod,
Nutrient Agar Clear
Blood Agar Clear
Eosin Methylene Blue Agar Clear
Mannitol Salt Agar Tan, Circles, Elevated
Glucose Acid Pro- Positive Gas Production- Negative
Sucrose Acid Pro-Positive Gas "- Neg
Lactose Acid "- Pos Gas "- Positive
Catalase productions- Negative
Urease Productions- Negative
Startch Utilizatoin- Absorbed Startch
dshenberger
Garter
Garter
 
Posts: 4
Joined: Tue Dec 02, 2008 8:35 pm


Return to Microbiology

Who is online

Users browsing this forum: No registered users and 0 guests