Login

Join for Free!
119308 members


isolate yeast

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

isolate yeast

Postby jakkaas1 » Sat Jun 14, 2008 1:12 pm

hello frnds i am doing project on alcohol n poly alcohol production by using yeast so pls can u provide some information

1)what r good source of obtaining yeast after honey?we r trying various fruits, so what should we do to optimize isolation of yeast

2)what media other than gye should i used.media of different composition

3)i tried to isolate yeast from skin of grapes but other fungus dominated, so what shoud i do to avoid then n any other helpful information u can provide.

thank u
jakkaas1
Garter
Garter
 
Posts: 3
Joined: Fri Jun 13, 2008 2:02 pm

Re: isolate yeast

Postby Xubu » Fri Jul 18, 2008 2:33 pm

Hi,

1) Honey is a good source for isolation of Xerotolerant Yeast (such as: Zygosaccharomyces..) which is widely used in fermentation of high concentrated solution. In my experiences, yeast occurs everywhere around us, so you can try other source, not only sources which contain sugar. Isolation sources also depend on which substrate you want to ferment and which poly alcohol you want to product.

2) In my lab, we used a very easily-prepared media - Malt Glucose (just consist of Malt and Glucose)

3) Yeasts grow quicker than fungus, so one of thing you can do to avoid fungus is to pick the yeast colonies out when the colonies of both fungus and yeasts is still small. Or you can try
Xubu
Garter
Garter
 
Posts: 2
Joined: Fri Jul 18, 2008 2:06 pm

Re: isolate yeast

Postby Bionic » Mon Nov 10, 2008 9:44 am

hello frnds i am doing project on alcohol n poly alcohol production by using yeast so pls can u provide some information

1)what r good source of obtaining yeast after honey?we r trying various fruits, so what should we do to optimize isolation of yeast

2)what media other than gye should i used.media of different composition

3)i tried to isolate yeast from skin of grapes but other fungus dominated, so what shoud i do to avoid then n any other helpful information u can provide.




1. i ever try on banana or sweet potatoes
2. PDA (potatoes Dextrose agar)
3. i think you have to sterilizate your media till 120 C and then give media antifungus as like nystatin and antibiotic as like streptomycin

i have how to make the media and complete with compotition of it.
Bionic
Garter
Garter
 
Posts: 4
Joined: Fri Nov 07, 2008 12:00 pm
Location: indonesia


Postby MrMistery » Mon Nov 10, 2008 7:44 pm

there are a whole bunch of yeast media out there. It really depends on what you are culturing.
About your third question: it is kind of tricky. First of all, you need to work sterile and add an antibiotic to your medium to make sure bacteria won't overrun your yeast. Second, I think that your grape skin are not going to contain one type of yeast, but more. If that is the case, then honestly you're fucked. Unless you can find something that your target yeast can do that others cannot(metabolise something, grow in the presence of something, grow in the absence of something etc). Your best bet would be to hope that you get exctly what you need and make sure you work sterile so that you don't get contaminants.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)


Return to Microbiology

Who is online

Users browsing this forum: No registered users and 2 guests