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more information Acrilamide vs agarose

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more information Acrilamide vs agarose

Postby alessandra » Thu Nov 06, 2008 3:02 pm

Hi I need some help

Someone can tell me about a book, article, where I can find the best use of acrylamide and agarose gel. I mean acrilamide gives high resolution, but I couldn't find more information about this.
thanks...
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Postby canalon » Fri Nov 07, 2008 2:51 am

What do you want know?
Wikipedia in electrophoresis will probably give you a good answer but in short:
-Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).
-Agarose is not as discrimant, but is good enough to discriminate samples with 20bp or more difference (depending on the size), easy to prepare, and harmless until stained. And cheaper to prepare.
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Postby Bionic » Fri Nov 07, 2008 1:10 pm

my teacher ever said to me.. depend on the object which our learned if we study abot DNA we use agarose and if protein or enzyme is acrylamide
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Re:

Postby canalon » Sat Nov 08, 2008 3:31 pm

Bionic wrote:my teacher ever said to me.. depend on the object which our learned if we study abot DNA we use agarose and if protein or enzyme is acrylamide


Although When working with DNA, agarose is used most of the time, for some applications, acrylamide is used. For example to run your gel in highly denaturing conditions (TGGE, DGGE), or to look at very short difference in size (sequencing)
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Re:

Postby MrMistery » Sun Nov 09, 2008 9:02 pm

canalon wrote:-Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).


Or you can just buy ready-cast :lol:
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Re: Re:

Postby canalon » Mon Nov 10, 2008 4:59 pm

MrMistery wrote:
canalon wrote:-Acrylamide gives a very good resolution but gels are long and complicated to prepare, expensive and use plenty of nasty chemicals (acrylamide is a neurotoxin).


Or you can just buy ready-cast :lol:


If you can afford it. Or if they fit your needs... But even agarose exist ready cast now... and some even include the EtBr. Good for those who can afford that kind of thing.
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Re: Re:

Postby MrMistery » Mon Nov 10, 2008 6:19 pm

canalon wrote:If you can afford it.


That's why I love Harvard :lol: :lol:
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