Urgent: info ELISA/EIA kit

[DoctorStein]

Hello,

I need ELISA/EIA kit to detect triiodothyronin (T3) level and the other one for thyroxin (T4) level of mouse and rat. It is easy to get ones for human, but so far I found difficulties to found the ones for mouse and rat. Hopefully I would get clue from you here.

Thanks in advance!

[blcr11]

Is rat or mouse T3 or T4 iodinated at different points or something? T3 and T4 are not proteins so, unless there is something chemically unique about them, I would think an assay for the human hormones would work just as well in rodents--all other things being equal, like, for example, there's no kind of rodent interfering molecule that scarfs up the human Elisa reagent, or some wierd thing like that.

[DoctorStein]

But from the package insert of regular insulin kit I got, it is said that the kit will result 0 % cross link with rat and mouse insulin? It seems to me that the kit doesn't work for rodents' insulin. Or it means something else? I'm sorry my English is limit.

[blcr11]

Yes, but insulin is a protein and the sequences of rat, human, and mouse insulin are different and may not be (apparently aren't) recognized by the Eliza antibodies. T3 and T4 are iodinated steroid-like molecules, not peptides or protein. There shouldn't be any difference in their structures from animal to animal--unless the points at which the iodine atoms are attached differs between species. I wouldn't think so, but I don't know that for a fact.

[MrMistery]

they aren't(according to my course on hormones)
T3: 3,3',5-triiodo-L-thyronine
T4: 3,3',5,5'-tetraiodothyronine
In all animals they appear.