sequence of steps for PCR, etc

[upinder1955]

What is the sequence of steps carried out for
1. PCR
2. cloning a gene
3. DNA finger-printing
4. taking out pure DNA from a cell
Please reply. It is urgent.

[canalon]

1-see for example: http://en.wikipedia.org/wiki/PCR
2- look up for the rest by yourself
3-come back with questions that show you have thought about your homework
4-receive help

Yes spoon feeding homework answers is not the site policy.

[mith]

It's urgent though. lol, better delegate it to the internets.

[pguo]

I think you are doing something right now, so it's urgent to know the protocols for your experiments. am I right?

You can find PCR and its related protocols over here http://www.lab-manual.com/lm_013.htm

There is another place you can find DNA, RNA, protein and their related, such as molecular cloning background and protocols. http://www.e-biotek.com/ebiotek_304.htm

In addition, you can easy find some ebooks and handbooks at above links. Let me know if I can help further.

Good luck.

[Anneitt]

hi , i'm a newcomer , it is the first time i come here. i'm engaged in biochemicals products , the main products are TMB series , such TMB-CL,TMB-S,-TMB-PS, it used in ELISA KITS ,and EIA KITS.
Biological buffers , such as HEPES,POPSO,MOPS,CAPS,CAPSO,CHES,MES,PIPES,TRIS,DIPSO,AMPSO,BICINE,etc. these are used in clinical diagnositc kits , cell culture , gene diagnosis , detection reagent, life science area.

If there is anything i can do for you , please feel free to contact with me . my email address is tcchemical_vivi@yahoo.com.cn .

Good luck!
Annie