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jelly fish protain

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jelly fish protain

Postby mehdi71000 » Thu Feb 22, 2007 10:39 pm

hi every body. how are you
there is a protain in the jelly fish that emits light (GFP). if i extract this protain from the cells. how can i make it emit light? i guess what im asking is how can that protain emit light. what chemicals must be present?
thanks it really helps.

is this protian also found in squid? :idea:
if no whats same question for the squid please. thanks
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Postby MrMistery » Thu Feb 22, 2007 11:35 pm

http://en.wikipedia.org/wiki/Green_fluorescent_protein
The protein only floresces green when hit by blue light, it does not generate light. Squid do not have it.
You might wanna look into luciferase. Luciferase oxidises a substrate and converts the released energy into light. Unlike GFP, luciferase actually "produces" the light
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Postby mehdi71000 » Fri Feb 23, 2007 12:09 am

thanks MrMistery
is there any other speacies beside fire fly that produce Luciferase or any other florecence producing protains.
thanks
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Postby MrMistery » Fri Feb 23, 2007 11:16 am

the luciferase enzyme has been "discovered" by bacteria, fungi, sea anemones, dinoflagelates and fireflies. Here is a link to a page describing luciferase. Very cool. I have actually printed all those files and made a molecule catalog
http://www.pdb.org/pdb/static.do?p=educ ... b78_1.html
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Postby mehdi71000 » Fri Feb 23, 2007 2:24 pm

thanks MrMistry it have opened my mind on many things
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Postby canalon » Fri Feb 23, 2007 9:17 pm

The luxCDABE operon can be isolated directly from Photorhabdus luminescens DNA. It gives luminescence with an enzymatic reaction (needs O2 and is Temperature dependant).

As for GFP you just need the right bulb to make it fluoresce.
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Postby MrMistery » Fri Feb 23, 2007 9:58 pm

just out of curiosity patrick, did you search or did you know the name of that operon by heart?
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Postby mehdi71000 » Fri Feb 23, 2007 10:32 pm

thanks canalon
is there a place were i can buy luxCDABE operon?
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Postby canalon » Tue Feb 27, 2007 4:21 am

_at_ Andrew:
I did not have to search because in one of my projects I have to work with a plasmid containing the lux operon.

@Medhi:
Don't ask, I cannot give you the strain, it come from another lab and we cannot share the strains, they have not published the construct. But it works.
If you can find it somewhere else? I would do a pubmed search with lux and E. coli as keywords and then contact researcher that have worked with such constructs if they exist.
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