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the effects of antibiotics on bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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the effects of antibiotics on bacteria

Postby ti gon » Thu May 19, 2005 12:00 pm

can somebody pls help me with my coursework. I ve 0nly got 2 days to do it. my teacher is very rigorous and i need to obtain a grade A to pass. the subject title is the effects of antibiotics on gram positive and gram negative bacteria. any sugestions???????? pleeeeez help ti gon. :(
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Postby MrMistery » Thu May 19, 2005 8:42 pm

All i know about this subject is that antibiotics stops the bacteria from dividing... Maybe Patrick knows some usefull sites for you...
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Postby canalon » Thu May 19, 2005 9:20 pm

I'd like to help but the question is a bit to vast to be answered here. There are books of more than 1000 of pages specifically treating the subject of which antibiotic is efficient against which kind of bacteria.

I found an interesting review on the subject here http://www.merck.com/mmhe/sec17/ch192/ch192a.html
and here:
http://pathmicro.med.sc.edu/mayer/antibiot.htm

It may help you start, but if you were more precise about what you need, or the limit of your subject I could give a more complete answer.

Patrick
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Postby ti gon » Fri May 20, 2005 12:02 pm

hiya yea sorry about the indistiction of my question. the bacterias i am using are ecoli and S.epidermis and the the antibiotics are ampicillin and Kanamycin. can you believe i have got to do a 100 pages on the subject??? its just absurd!! :evil: i practically begged for an extension and i got a 2 day extension. :x i really need to get this completed and i think with the help of my new friends patrick and Mr Mistry i hope i will have it done :wink: thanks alot pal XXXXXXXxxxx
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Postby MrMistery » Fri May 20, 2005 7:51 pm

Patrick is the one you should thank. I am just in the dark on this subject as you are.
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Postby ti gon » Mon May 23, 2005 12:07 pm

YES. BUT YOU SUGGESTED I GET PATRICKS HELP. SO REALLY THANK YOU!
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Postby MrMistery » Mon May 23, 2005 9:05 pm

Lol... Welcome then :D :D :D :D
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Postby canalon » Tue May 24, 2005 1:32 pm

Sorry but I come back from a long week-end. I do really not see how you could do 100 pages on the subject with only 2 genus of bacteria and 2 antibiotics! Sure it is not 10 pages?

But what you could do:

- Determination of the minimal inhibitory concentration (MIC) of both antibiotics on both bacteria: Find a medium that is suitable for both (Mueller-Hinton is usually the one recommended, but I'm sure wether S. epidermis would grow on it) either agar plates or broth. Prepare different concentration of antibiotic and inoculate with approximately 10^6 bacteria per ml. Grow @37C for 16 to 20h and note which concentration inhibits growth. (in case of broth make triplicate experiment). Compare MIC for both species and discuss.
-Use Medline and the links I gave you and try to identify the targets of your antibiotics to compare the composition of the targets for both species.
- Have a lot of illustrations :lol:

Back in the lab for further questions. Good Luck

Patrick
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Postby ti gon » Wed May 25, 2005 9:59 am

NO PATRICK ITS NOT TEN ITS A HUNDRED ALRIGHT. BUT I HAVE FINALLY COMPLETED IT AND GUESS WOT????//// I GOT MY GRADE!!!!! WOOHOO!!!!! ALL THANKS TO YOU PAT. ANYWAY GO ANYWHERE NICE ON YOUR LONG WEEKEND????
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Postby MrMistery » Wed May 25, 2005 8:01 pm

Congratulations!!
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Postby Poison » Thu May 26, 2005 4:19 pm

Happy to hear that ti gon...
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Postby canalon » Thu May 26, 2005 5:12 pm

Good for you. I don't know how you managed that, I wouldn't have been able to...

Congratulations
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