help needed - HUVEC-C cells

[jmc2]

Hello everyone,

I am doing some research on RGD targeting using Human Umbilical Endothelial Cell Line - C (HUVEC-C).

The growth medium I am is DMEM with 20 % FCS, 0.1 mg/ml heparin, 0.05 mg/ml mitogen (endothelial cell growth supplement) and 1 % Penicillin-streptomycin. The cells grow quite slowly. I am wondering if anyone can give me some tips or suggestions on culturing HUVEC cells.

Also, I heard HUVEC can only be used within 10 passages? I don’t know if this is true? As the HUVEC I got are passage 9 and 14 already.

Thank you for replying to both questions in advance!

JMC2

[sdekivit]

14 passages is too old for HUVEC cells

[wheatonhgc]

HUVEC!!!! Hi All, i have been having a lot of trouble culturing HUVEC-C into confluency... I ordered the cells from ATCC. I modified the F-12 Kaigh's medida with 10%fetal bovine serum, 0.8% penicillin, 0.1mg/ml heparin, and 0.05 mg/ml ECGS.. the cells are incubated at 37C with 5%CO2.... I don't know why my cells died within two days. Please help me out if you know the cause of cell death or if you know who is the expert on this cell type, please email me at [email protected]