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Do bacteria have S-S linking mechanism?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Do bacteria have S-S linking mechanism?

Postby MrMistery » Sun Nov 19, 2006 11:58 am

OK, so if you want to make fully functional humuline you need it to have the right 3d structure right? this means you need the bacteria to create the propper disulfide bridges, right?
my question is: do you insert the gene that codes for the disulfidisomerase enzyme in the bacteria along with the insulin gene, or do bacteria have them on their own? and another thing: if they do have them, how come these enzymes do not interfere with the normal proteins that do not need disulfid bridges, although they are possible to create(disulfidisomerases, from what i know, are very unspecific in the formation of bonds, and go by a trial-and-error mechanism)because bacteria don't have an ER to trap the enzyme there like eukaryotes...

I've asked this question to many people and never got a complete and trustworthy answer
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Re: Do bacteria have S-S linking mechanism?

Postby zami'87. » Fri Nov 27, 2009 7:15 am

This? dsbC- Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein. Also acts as a disulfide isomerase.
http://www.uniprot.org/uniprot/P0AEG6

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Postby JackBean » Fri Nov 27, 2009 8:03 am

LOL :lol: quite old thread :)
The cytoplasm of bacteria is rather reducing enviroment, so if you wish oxidized SS bridge, you must target your protein into periplasm, as zami mentioned :)
http://www.biolib.cz/en/main/

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Re:

Postby zami'87. » Fri Nov 27, 2009 10:24 am

JackBean wrote:LOL :lol: quite old thread :)


yes,so old..it had been posted before virtual egg and virtual sperm fused and gave JackBean profile in our virtual community :lol:
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Postby JackBean » Sat Nov 28, 2009 1:06 pm

:lol:
Well, it is sooo old, that at that time I even didn't almost know, what biochemistry or bacteria are :lol:
http://www.biolib.cz/en/main/

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