Re: Denaturing agents to convert d.s Lambda DNA to s.s DNA

[shankargan2006]

Hi everybody,
It would be really great if someone could answer the following questions.

1.What is the largest commercially available Single Stranded DNA (that’s much longer than the M13 (7249 bp) DNA)

2.What are the denaturing agents that can be used to denature a double strand (e.g. Lambda DNA) to get a single strand, preserving streptavidin-biotin bonds? Also what’s the ideal pH and salt concentration to keep the single strand intact after the double stranded DNA was denatured?


Any kind of suggestions are welcome.
Look forward to your replies.

Shankar