Login

Join for Free!
118246 members


DNA/RNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

DNA/RNA

Postby Diana Timpano » Sun Apr 24, 2005 3:56 pm

Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?
Diana Timpano
Garter
Garter
 
Posts: 11
Joined: Thu Mar 24, 2005 6:33 pm

Re: DNA/RNA

Postby Arty » Sun Apr 24, 2005 4:59 pm

Diana Timpano wrote:Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?


Pretty easy!!
No of amino acids= 300/3 = 100 amino acids
No of nucleotides= 150*3 = 450 nucleotides
Arty
Garter
Garter
 
Posts: 19
Joined: Sun Apr 10, 2005 11:40 am

Re: DNA/RNA

Postby zami'87. » Sun Apr 24, 2005 7:51 pm

Arty wrote:
Diana Timpano wrote:Hi....A couple of questions..help....

If a structural gene contains 300 DNA nucleotides, how many amino acids will be used in the protein synthesis process?

If a protein has 150 amino acids, how many DNA nucleotides would make up the structural gene?


Pretty easy!!
No of amino acids= 300/3 = 100 amino acids
No of nucleotides= 150*3 = 450 nucleotides

if we don't go in details(stop codon...)?
User avatar
zami'87.
Coral
Coral
 
Posts: 257
Joined: Sat Mar 05, 2005 6:56 pm
Location: Serbia


Postby biostudent84 » Sun Apr 24, 2005 9:02 pm

448 maximum if you include the start codon and stop codons.
User avatar
biostudent84
Site Admin
Site Admin
 
Posts: 974
Joined: Thu Nov 11, 2004 6:00 am
Location: Farmville, VA

Postby canalon » Sun Apr 24, 2005 9:09 pm

biostudent84 wrote:448 maximum if you include the start codon and stop codons.


I think if you really want to count stop codons (start codons are transcribed) you get these results
(300 nt - 1 non transcribed stop codon)= 99 aa
and (150aa + 1 non transcribed stop codon)x3= 453nt

Patrick
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby biostudent84 » Mon Apr 25, 2005 2:47 am

What are the "n" and the "t" meaning in this example?
User avatar
biostudent84
Site Admin
Site Admin
 
Posts: 974
Joined: Thu Nov 11, 2004 6:00 am
Location: Farmville, VA

Postby canalon » Mon Apr 25, 2005 3:25 am

biostudent84 wrote:What are the "n" and the "t" meaning in this example?


oopps "nt" stands for nucleotides (bases) as "aa" stands for amino acids. :oops:

Patrick
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby biostudent84 » Mon Apr 25, 2005 1:32 pm

I'd like to know where I got that number from and what the heck I was thinking..... :( :( :( :( :(

98 maximum if you count the start and stop codons.

Sorry.....
User avatar
biostudent84
Site Admin
Site Admin
 
Posts: 974
Joined: Thu Nov 11, 2004 6:00 am
Location: Farmville, VA

Postby canalon » Mon Apr 25, 2005 2:17 pm

biostudent84 wrote:I'd like to know where I got that number from and what the heck I was thinking..... :( :( :( :( :(

98 maximum if you count the start and stop codons.

Sorry.....


:!: Start codons ARE transcribed, hence 99.
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby biostudent84 » Mon Apr 25, 2005 5:16 pm

They are? Thanks
User avatar
biostudent84
Site Admin
Site Admin
 
Posts: 974
Joined: Thu Nov 11, 2004 6:00 am
Location: Farmville, VA

Postby MrMistery » Wed Apr 27, 2005 8:12 pm

From what i know, start codons are transcripted, but if your protein does not begin with metionin then it is later removed
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

INTRONS

Postby cytochromeP » Thu May 05, 2005 3:33 pm

r we forgetting something...
Oh yes...INTRONS
if the structural gene contains introns then the no. of aa reqd. for protein making is indeterminate !
or else if its a bacterial gene then my predecessors have already done the math.
Know thyself.
User avatar
cytochromeP
Death Adder
Death Adder
 
Posts: 73
Joined: Wed Apr 06, 2005 2:17 pm
Location: Third Rock From The Sun

Next

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 5 guests