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Catalase

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Catalase

Postby mexicoDFA » Fri Apr 22, 2005 12:58 am

I know catalase is an enzyme that helps turn H2O2 into H2O and O2. Im doing an experiment on pH levels and enzymes, and I cant figure out how to indicate wether or not the reaction took place. Any help would be appreciated.
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Postby mith » Fri Apr 22, 2005 2:17 am

you should see bubbles(o2 gas)
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Re: Catalase

Postby zami'87. » Fri Apr 22, 2005 8:12 am

maybe go to chemistry forum : http://www.chemicalforums.com
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Postby James » Fri Apr 22, 2005 3:18 pm

Use a manometer to see if, and how fast the O2 is produced
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Re: Catalase

Postby TomMol » Sat Apr 30, 2005 1:42 pm

mexicoDFA wrote:I know catalase is an enzyme that helps turn H2O2 into H2O and O2. Im doing an experiment on pH levels and enzymes, and I cant figure out how to indicate wether or not the reaction took place. Any help would be appreciated.



I use Merck strips to indicate H2O2 concentration in solution during reaction. It`s very good method.
Good luck

Tom

tgosiews@cm-uj.krakow.pl

Ps. Do you know pseudocalalase (manganese catalase)?
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catalase

Postby VickY » Sun May 08, 2005 2:52 pm

1. measure OD at 240 nm of H2O2 disappearing
2. measure production of O2 by oxyden electrode, as we do in our laboratory.
good luck :wink:
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