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screening for bacteria from mucous of seahorses.

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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screening for bacteria from mucous of seahorses.

Postby julius » Thu Jul 13, 2006 4:46 pm

hi to all...

How am I suppose to do this kind of title?
I am looking for the potential bacteria from the mucous layer of the seahorses, that can be used in pharmaceutical sector.

Then, i have to characterize the bacteria due to their pathogenic and antigenic properties.

Lastly, determine the sensitivity of the microbs towards broad and narrow spectrum antibiotics.

which type of methods and meterial should i use? I am quite new in this field, please lend me a hand.

Please comment on my title also,thanks...
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Postby mkwaje » Fri Jul 14, 2006 3:11 am

Wow.. you've got a lot of work ahead of you... and your results will be very interesting. I don't have much experience in screening from seahorse mucous. Why did you choose seahorses btw... It would be challenging to cultivate those bacteria from seahorse mucous though. I assume they would be a natural microflora from seahorses and you have to simulate those conditions in your media to be able to cultivate them in vitro. Many bacteria have developed dependency on their symbiont host thus making them difficult to cultivate in plates / broths. Take care of media factors like the appropriate salt concentrations, pH, and temp. I think you can try using seahorse mucous as part of the media, hmmm. Well, after you have successfully isolated some of these bacteria, you have to identify them. Regarding pathogenic properties and antibiotic sensitivity testing, I can't see their purpose unless you are isolating these pathogens from sick seahorses. Are you trying to look for antibiotic resistant bacteria infecting sea horses? Assuming the end result that you want is an antimicrobial for the pharma industry, I think you can probably streak it in media growing different pathogens and look for zones of inhibition. Of course, it won't work if your isolates require special media to grow. You can then try paper disc or cylinder cup assay later using extracts. But then, the most important thing is obtaining numerous isolates first - that is challenging enough.

Good luck on your research. I just quit my teaching job and will try pharma research too. Basic research is expensive though but the rewards are great if you're lucky though.
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Postby julius » Sat Jul 15, 2006 2:06 pm

Thanks for the suggested methods and ideas. The reason I choose this title is there is still not much of people did the research on seahorses in M'sia. Yes, i am moving in pharma direction. I am not taking the pathogens, I just want the antimicrobial from the seahorse mucous.
Do u have any journal or any project that is closely related to my title? i am trying to compare my methods with the others also. Btw, can i ask what u been teaching ? Is it something related to microbiology? if yes, can i refer to you in the future?
thanks...
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Postby mkwaje » Sun Jul 16, 2006 10:05 am

I did identification and sensitivity testing in my college days in a pharmaceutical company as practicum work. I taught laboratory courses in Microbial genetics, basic MCB, advanced techniques in MCB, environmental MCB, and food MCB. And also some basic Zoo and chem subjects.

I did some isolation of thermophilic bacteria from volcanic mud springs and hot springs. I plan to screen them for useful enzymes and antimicrobials. Due to power outages, I wasn't able to maintain my cultures. Couldn't mantain the incubator at 65-80 C. So basically, I was able to isolate my thermophiles (after much optimization of culture media - took me about a year) and isolated some DNA. Your work with shrimps would be different but you have to simulate the shrimp environment to successfully isolate and maintain stable bacterial cultures..

Best of luck...
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