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E. coli strain for plasmid prep AND expression

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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E. coli strain for plasmid prep AND expression

Postby vernet » Wed Dec 08, 2004 4:46 pm

I am looking for an E. coli strain for efficient transformation (recA) AND good expression of gene under lac promotor that can also deliver good quality plasmids. Any hints?
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Postby biostudent84 » Wed Dec 08, 2004 5:47 pm

Usually, you can kill off all bacteria that are NOT part of your experiment. You might have one or two left, but let them divide a few times, and you can quickly come up with a good culture.
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