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Transcription / translation rate, nucleases and degradation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Transcription / translation rate, nucleases and degradation

Postby neon » Tue May 30, 2006 8:06 am

Hello,

I am looking for models of transcription and translationregulation - more specifically, any equations that are proposed to define transcription or translation rate. Is anyone familiar with this? I understand that we can consider transcription degradation as a function of nuclease activity and transcript concentration - is this correct?

Are nucleases specific in what they act on? i.e. will a specific mRNA transcript be degraded only by a specific endonuclease, or will any endonuclease degrade any mRNA molecule it comes across? What is the case for proteases?

Millions of thanks in advance :-)

Neon
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Postby tranjo » Sat Jun 03, 2006 3:49 pm

I think there are mathematical models for what you speak of; however, they're almost useless because they are for closed systems - thus not really coming close to the dynamics of a cell (you should major in systems biology).

I don't know the answer to your transcript degradation function question, but it sounds correct for what you'd find in the cytoplasm.

I believe that a nuclease that destroys RNAs will attack almost any RNA it comes across. How specific transcripts are destroyed is still up for debate, I think. There are examples of targeted degradation with microRNAs and NMD, but that doesn't account for the whole mill. Most transcripts that are targeted for destruction get "shipped" to places, like the P-bodies during the cell cycle. Proteases are not specific for a particular protein, but are particular for amino acid sequences. Like Serine proteases. I think there are exceptions to everything I just wrote, but I hope it helps!

Best, J
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