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enzyme catalase

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enzyme catalase

Postby taylor's team » Mon May 08, 2006 9:33 pm

:? i have conducted an experiment on the enzyme catalase. liver, kidney, heart and muscle were used. The kidney had the highest rate of reaction but the liver should of being the highest as it contains more catalase. can any one explain why this result was obtained. this experiment was achieved usin hydrogen peroxide. please please help thankyou xx LT
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Postby canalon » Tue May 09, 2006 12:21 am

I looked at your protocol inmy cristal ball, and I saw you making mistae in your dilutions.
Maybe it's wrong (it's a cheap model "made in China") but I had to rely on it considering the whole lot of information that you gave us....
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Postby zonia30 » Tue May 09, 2006 6:22 pm

well taylors team i struggled myself with this one probably just because the kidney may have been slightly more active than the liver i'm guessing both kidney & liver reactions were alot higher than the heart and muscle as both those organs are more efficient in terms of producing less waste. hope that helps just call zonia30 haha
dont forget this site in your bibliography or LT will have ya !!!!! lol
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Postby taylor's team » Tue May 09, 2006 7:43 pm

:lol: Thanx Zonia30 I am still bloody confused as I am still on intro lol but I am gettin there slowly but surely. If I have 2 put this in bib then so do u haha. See u 2moz xx
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Postby victor » Wed May 10, 2006 3:25 pm

Canalon wrote:I looked at your protocol inmy cristal ball, and I saw you making mistae in your dilutions.
Maybe it's wrong (it's a cheap model "made in China") but I had to rely on it considering the whole lot of information that you gave us....


Made in China?? As a Chinnese, I'd also agree with you Canalon even though I don't know why their quality are so bad... :lol:
For those kidney thing, I can say that, beside catalase enzyme, there're some substances in the kidney that also can react with H2O2. So, the result is that kidney seems like have more catalase compared with liver..
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Postby taylor's team » Wed May 10, 2006 10:41 pm

i think maybe........ the piece that was cut from the kidney was more active than the piece from the liver, maybe the liver had more blood vessels in that cut off than the kidney ia m on the right lines???? Thanx LT x
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Postby canalon » Thu May 11, 2006 12:03 am

I am not even starting to make any assumptions without seeing the protocol...
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Postby victor » Thu May 11, 2006 11:57 am

I'm not pointing into comparison of their blood vessels...but, as you see that H2O2 is a reactive oxydator that can easily get reducted into O2 and water if it meets ceertain molecules....
I can think that it's related with amine compounds in the kidney. Um, what part of kidney that you use as a H2O2 tester? cortex, medulla or pelvis??
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